Organelle Biogenesis and Homeostasis
- GROUP LEADER:
- Pedro Carvalho
- POSTDOCTORAL FELLOWS:
- Ombretta Foresti, Montserrat Serra, Gabriel E Mora
- PhD STUDENTS:
- Alexandra Grippa, Annamaria Ruggiano, Lisa Johnsen, Victoria Rodríguez , Nivedita Natarajan (started September)
- Laura Buxó
The endoplasmic reticulum (ER) is the primary site for the biogenesis of membrane and secreted proteins. Folding and maturation of these proteins is an elaborate process, frequently involving a number of posttranslational modifications (like disulphide bonds, glycosylation, lipidation, etc.) and/or assembly into protein complexes. Most of the cellular lipids are also synthesized at the ER. Perturbations in any of these processes often result in ER stress, a hallmark of many diseases such as diabetes, obesity and cancer. Our long-term goal is to understand how these multiple functions of the ER are coordinated and integrated under different physiological conditions.
- Mechanisms of recognition and degradation of misfolded proteins by ERAD
- Identification of novel regulated ERAD substrates
- Regulation of sterol homeostasis by ERAD
- Novel mechanisms of protein quality control in the ER
- Quality control of inner nuclear membrane proteins
- Mechanisms of lipid droplet biogenesis at the ER
- Mechanisms of protein targeting to lipid droplets
Foresti O, Rodriguez-Vaello V, Funaya C, Carvalho P.
“Quality control of inner nuclear membrane proteins by the Asi complex.”
Science, 346(6210):751-5 (2014).
Stein A, Ruggiano A, Carvalho P, Rapoport TA.
“Key steps in ERAD of luminal ER proteins reconstituted with purified components.” Cell, 158(6):1375-88 (2014).
Ruggiano A, Foresti O, Carvalho P.
“Quality control: ER-associated degradation: protein quality control and beyond.”
J Cell Biol, 204(6):869-79 (2014). Review.